Sleep-Enhancing Effect of Water Extract from Jujube (Zizyphus jujuba Mill.) Seeds Fermented by Lactobacillus brevis L32

Although Ziziphus jujuba Mill (jujube) is used in folk medicine for hypnotic sedative, anxiolytic, and many other purposes, to date, only a few studies have revealed its sleep-promoting effects related mechanisms. Currently, drugs the treatment of sleep disorders various side effects, so it essential develop safe natural materials. Therefore, we evaluated sleep-enhancing activity mechanism action an aqueous extract jujube seeds (ZW) fermented with Lactobacillus brevis L-32 rodent models. The starch contained ZW was removed by enzymatic degradation L. obtain product (ZW-FM) high γ-aminobutyric acid (GABA) content. To evaluate effect ZW-FM, pentobarbital-induced tests were performed on ICR mice, electroencephalography analysis undertaken Sprague Dawley rats. Additionally, awakening relief ZW-FM confirmed caffeine-induced insomnia model. Finally, enhancement analyzed using GABA receptor type A (GABAA) antagonists. ZW-FM-treated groups (100 150 mg/kg) showed increased time, especially δ-wave time during non-rapid eye movement (NREM) sleep. In addition, mg/kg group decreased latency particular, NREM REM which caffeine treatment, treatment. ZW-FM-induced increase inhibited GABAA antagonists picrotoxin, bicuculline, flumazenil, confirming that result GABAergic mechanism. These results strongly suggest water from acts as compound binding.